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The Much-Needed Technology for Pathogen Testing

By Food and Beverages | Monday, September 21, 2020

As unique as it sounds, LAMP technology has brought the benefit of a single access protocol for all pathogens that enable technicians to leverage the same familiar process for every tests. The workflow involves a very minimal accessory that simplifies the process and also reduces the risk of human-caused error.

FREMONT, CA: The realization of the industry’s appetite for a pathogen detection solution closer to the real-time simplicity and speed experienced with ATP testing, scientists have offered a solution called the LAMP. The LAMP system allows the food safety technicians to generate more than a billion exact copies of target DNA from their samples as soon as 15 minutes into imitation.

No More Thermal Cycling Requirement:

Thermocyclers, the support system of polymerase chain reaction (PCR) tests often alter the temperature to facilitate a reaction. In a way for every food and beverage, or environmental surface sample examined the PCR system undergoes multiple phases of heating up, cooling down, and then re-warming. It is essential to incorporate internal amplification control (IAC) components to alleviate the hindrances in detecting the pathogen under investigation and eliminating the chances of false-negative results. To reduce the delays and errors, LAMP amplifies DNA isothermally at a constant and stable temperature range. The consistent amplification brings better tolerates to the sample matrix inhibitors and helps to house the technology in a smaller machine with lesser space on the workbench.

DNA Primers and The Missing Loop:

Older PCR tests identify only two separate regions of the target genetic material and rely on them to anneal separated DNA strands of it, later they copy and amplify it. On the other hand, LAMP technology utilizes four to six primers, which can recognize six to eight different regions from the sample’s DNA. The primers used not only cause the DNA strand to relocate but loop the end of it collectively before getting amplified. The exclusive looped structure accelerates the reaction and improves test result sensitivity by allowing an exponential accumulation of the target DNA.

Reduction of Steps:

Technicians need to enrich their samples to grow microorganisms to detectable levels before any genetic amplification takes place. The commercialized LAMP assay kit offers a more ready-to-use approach that does not necessarily need any buffers or reagent mixes to organize a sample. Lab technicians shift their enriched sample to the lysis tube, heat and cool it, and then pipette it to another cell before performing the test with the 3M Molecular Detection System.

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